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Issue 12, 2008
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An integrated microfluidic platform for sensitive and rapid detection of biological toxins

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Abstract

Towards designing a portable diagnostic device for detecting biological toxins in bodily fluids, we have developed microfluidic chip-based immunoassays that are rapid (< 20 minutes), require minimal sample volume (<10 µL) and have appreciable sensitivity and dynamic range (µM–pM). The microfluidic chip is being integrated with miniaturized electronics, optical elements, fluid-handling components, and data acquisition software to develop a portable, self-contained device. The device is intended for rapid, point-of-care (and, in future, point-of-incident) testing in case of an accidental or intentional exposure/intoxication to biotoxins. Detection of toxins and potential host-response markers is performed using microfluidic electrophoretic immunoassays integrated with sample preconcentration and mixing of analytes with fluorescently labeled antibodies. Preconcentration is enabled by photopolymerizing a thin, nanoporous membrane with a MW cut-off of ∼10 kDa in the sample loading region of the chip. Polymeric gels with larger pores are located adjacent to the size exclusion membrane to perform electrophoretic separation of antibody–analyte complex and excess antibody. Measurement of the ratio of bound and unbound immune-complex using sensitive laser-induced fluorescence detection provides quantitation of analyte in the sample. We have demonstrated electrophoretic immunoassays for the biotoxins ricin, Shiga toxin I, and Staphylococcal enterotoxin B (SEB). With off-chip mixing and no sample preconcentration, the limits of detection (LOD) were 300 pM for SEB, 500 pM for Shiga toxin I, and 20 nM for ricin. With a 10 min on-chip preconcentration, the LOD for SEB is <10 pM. The portable device being developed is readily applicable to detection of proteinaceous biomarkers of many other diseases and is intended to represent the next-generation diagnostic devices capable of rapid and quantitative measurements of multiple analytes simultaneously.

Graphical abstract: An integrated microfluidic platform for sensitive and rapid detection of biological toxins

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Publication details

The article was received on 01 Sep 2008, accepted on 13 Oct 2008 and first published on 24 Oct 2008


Article type: Paper
DOI: 10.1039/B815152K
Citation: Lab Chip, 2008,8, 2046-2053
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    An integrated microfluidic platform for sensitive and rapid detection of biological toxins

    R. J. Meagher, A. V. Hatch, R. F. Renzi and A. K. Singh, Lab Chip, 2008, 8, 2046
    DOI: 10.1039/B815152K

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