Jump to main content
Jump to site search

Issue 12, 2008
Previous Article Next Article

Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP

Author affiliations

Abstract

The study of cellular responses to changes in the spatial distribution of molecules in development, immunology and cancer, requires reliable methods to reproduce in vitro the precise distributions of proteins found in vivo. Here we present a straightforward method for generating substrate-bound protein patterns which has the simplicity required to be implemented in typical life science laboratories. The method exploits photobleaching of fluorescently tagged molecules to generate patterns and concentration gradients of protein with sub-micron spatial resolution. We provide an extensive characterization of the technique and demonstrate, as proof of principle, axon guidance by gradients of substrate-bound laminin peptide generated in vitro using LAPAP.

Graphical abstract: Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Aug 2008, accepted on 03 Oct 2008 and first published on 17 Oct 2008


Article type: Communication
DOI: 10.1039/B813897D
Citation: Lab Chip, 2008,8, 2164-2167
  •   Request permissions

    Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP

    J. M. Bélisle, J. P. Correia, P. W. Wiseman, T. E. Kennedy and S. Costantino, Lab Chip, 2008, 8, 2164
    DOI: 10.1039/B813897D

Search articles by author

Spotlight

Advertisements