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Issue 10, 2008
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Microfluidic patterning of nanodisc lipid bilayers and multiplexed analysis of protein interaction

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Abstract

We report a microfluidic method for precisely patterning lipid bilayers and a multiplexed assay to examine the interaction between the lipids and protein analytes. The lipids were packaged into nanoscale lipid bilayer particles known as Nanodiscs and delivered to surfaces using microfluidic channels. Two types of lipids were used in this study: biontinylated lipids and phosphoserine lipids. The deposition of biotinylated lipids on a glass surface was confirmed by attaching streptavidin coated quantum dots to the lipids, followed by fluorescent imaging. Using this multiplexed grid assay, we examined binding of annexin to phosphoserine lipids, and compared these results to similar analysis performed by surface plasmon resonance.

Graphical abstract: Microfluidic patterning of nanodisc lipid bilayers and multiplexed analysis of protein interaction

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Publication details

The article was received on 21 Apr 2008, accepted on 25 Jun 2008 and first published on 04 Aug 2008


Article type: Paper
DOI: 10.1039/B806733C
Citation: Lab Chip, 2008,8, 1723-1728
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    Microfluidic patterning of nanodisc lipid bilayers and multiplexed analysis of protein interaction

    E. D. Goluch, A. W. Shaw, S. G. Sligar and C. Liu, Lab Chip, 2008, 8, 1723
    DOI: 10.1039/B806733C

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