Biocompatible surfactants for water-in-fluorocarbon emulsions

C. Holtze; A. C. Rowat; J. J. Agresti; J. B. Hutchison; F. E. Angilè; C. H. J. Schmitz; S. Köster; H. Duan; K. J. Humphry; R. A. Scanga; J. S. Johnson; D. Pisignano; D. A. Weitz

doi:10.1039/B806706F

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Lab on a Chip

Issue 10, 2008

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Biocompatible surfactants for water-in-fluorocarbon emulsions

C. Holtze , A. C. Rowat , J. J. Agresti , J. B. Hutchison , F. E. Angilè , C. H. J. Schmitz , S. Köster , H. Duan , K. J. Humphry , R. A. Scanga , J. S. Johnson , D. Pisignano and D. A. Weitz

Lab Chip, 2008, 8, 1632-1639

DOI: 10.1039/B806706F
Received 21 Apr 2008, Accepted 25 Jul 2008
First published on the web 02 Sep 2008

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Drops of water-in-fluorocarbon emulsions have great potential for compartmentalizing both in vitro and in vivo biological systems; however, surfactants to stabilize such emulsions are scarce. Here we present a novel class of fluorosurfactants that we synthesize by coupling oligomeric perfluorinated polyethers (PFPE) with polyethyleneglycol (PEG). We demonstrate that these block copolymer surfactants stabilize water-in-fluorocarbon oil emulsions during all necessary steps of a drop-based experiment including drop formation, incubation, and reinjection into a second microfluidic device. Furthermore, we show that aqueous drops stabilized with these surfactants can be used for in vitro translation (IVT), as well as encapsulation and incubation of single cells. The compatability of this emulsion system with both biological systems and polydimethylsiloxane (PDMS) microfluidic devices makes these surfactants ideal for a broad range of high-throughput, drop-based applications.

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