Crossing microfluidic streamlines to lyse, label and wash cells

Keith J. Morton; Kevin Loutherback; David W. Inglis; Ophelia K. Tsui; James C. Sturm; Stephen Y. Chou; Robert H. Austin

doi:10.1039/B805614E

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Lab on a Chip

Issue 9, 2008

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Crossing microfluidic streamlines to lyse, label and wash cells

Keith J. Morton , Kevin Loutherback , David W. Inglis , Ophelia K. Tsui , James C. Sturm , Stephen Y. Chou and Robert H. Austin

Lab Chip, 2008, 8, 1448-1453

DOI: 10.1039/B805614E
Received 15 Apr 2008, Accepted 02 Jul 2008
First published on the web 23 Jul 2008

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We present a versatile method for continuous-flow, on-chip biological processing of cells, large bio-particles, and functional beads. Using an asymmetric post array in pressure-driven microfluidic flow, we can move particles of interest across multiple, independent chemical streams, enabling sequential chemical operations. With this method, we demonstrate on-chip cell treatments such as labeling and washing, and bacterial lysis and chromosomal extraction. The washing capabilities of this method are particularly valuable because they allow many analytical or treatment procedures to be cascaded on a single device while still effectively isolating their reagents from cross-contamination.

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Keith J. Morton
Kevin Loutherback
David W. Inglis
Ophelia K. Tsui
James C. Sturm
Stephen Y. Chou
Robert H. Austin

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