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Issue 9, 2008
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Crossing microfluidic streamlines to lyse, label and wash cells

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Abstract

We present a versatile method for continuous-flow, on-chip biological processing of cells, large bio-particles, and functional beads. Using an asymmetric post array in pressure-driven microfluidic flow, we can move particles of interest across multiple, independent chemical streams, enabling sequential chemical operations. With this method, we demonstrate on-chip cell treatments such as labeling and washing, and bacterial lysis and chromosomal extraction. The washing capabilities of this method are particularly valuable because they allow many analytical or treatment procedures to be cascaded on a single device while still effectively isolating their reagents from cross-contamination.

Graphical abstract: Crossing microfluidic streamlines to lyse, label and wash cells

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Publication details

The article was received on 15 Apr 2008, accepted on 02 Jul 2008 and first published on 23 Jul 2008


Article type: Paper
DOI: 10.1039/B805614E
Citation: Lab Chip, 2008,8, 1448-1453
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    Crossing microfluidic streamlines to lyse, label and wash cells

    K. J. Morton, K. Loutherback, D. W. Inglis, O. K. Tsui, J. C. Sturm, S. Y. Chou and R. H. Austin, Lab Chip, 2008, 8, 1448
    DOI: 10.1039/B805614E

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