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Issue 9, 2008
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On-chip screening method for cell migration genes based on a transfection microarray

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Abstract

Cell migration plays a major role in a variety of biological processes and a detailed understanding of associated mechanisms should lead to advances in the medical sciences, for example, in drug discovery for cancer therapy. However, the traditional methods used for analysis of cell migration cannot easily be scaled up for high-throughput screening. In this study, we have attempted to develop a novel simple method for high-throughput phenotypic screening for the identification of genes that are required for cell migration. As the appropriate cell line for the method, we found NBT-L2b cells that would be suitable for screening of migration-related genes in our method without influence by other cellular processes. Moreover, the idea for printing both the labeled fibronectin, for identification of the starting region of a cell, and the green fluorescent protein (GFP) expression vector, for identification of cells that had been transfected with siRNA and of the end point of migration, brings a rapid and efficient high-throughput screening procedure. Our new method will lead to an enhanced understanding of cell migration.

Graphical abstract: On-chip screening method for cell migration genes based on a transfection microarray

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Publication details

The article was received on 06 Mar 2008, accepted on 16 Jun 2008 and first published on 04 Aug 2008


Article type: Paper
DOI: 10.1039/B803879A
Citation: Lab Chip, 2008,8, 1502-1506
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    On-chip screening method for cell migration genes based on a transfection microarray

    R. Onuki-Nagasaki, A. Nagasaki, K. Hakamada, T. Q. P. Uyeda, S. Fujita, M. Miyake and J. Miyake, Lab Chip, 2008, 8, 1502
    DOI: 10.1039/B803879A

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