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Issue 9, 2008
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Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules

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Abstract

Motor-driven cytoskeletal filaments are versatile transport platforms for nanosized cargo in molecular sorting and nano-assembly devices. However, because cargo and motors share the filament lattice as a common substrate for their activity, it is important to understand the influence of cargo-loading on transport properties. By performing single-molecule stepping assays on biotinylated microtubules we found that individual kinesin-1 motors frequently stopped upon encounters with attached streptavidin molecules. Consequently, we attribute the deceleration of cargo-laden microtubules in gliding assays to an obstruction of kinesin-1 paths on the microtubule lattice rather than to ‘frictional’ cargo-surface interactions. We propose to apply this obstacle-caused slow-down of gliding microtubules in a novel molecular detection scheme: Using a mixture of two distinct microtubule populations that each bind a different kind of protein, the presence of these proteins can be detected via speed changes in the respective microtubule populations.

Graphical abstract: Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules

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Publication details

The article was received on 03 Mar 2008, accepted on 03 Jul 2008 and first published on 30 Jul 2008


Article type: Paper
DOI: 10.1039/B803585G
Citation: Lab Chip, 2008,8, 1441-1447
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    Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules

    T. Korten and S. Diez, Lab Chip, 2008, 8, 1441
    DOI: 10.1039/B803585G

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