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Issue 7, 2008
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Drop-based microfluidic devices for encapsulation of single cells

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Abstract

We use microfluidic devices to encapsulate, incubate, and manipulate individual cells in picoliter aqueous drops in a carrier fluid at rates of up to several hundred Hz. We use a modular approach with individual devices for each function, thereby significantly increasing the robustness of our system and making it highly flexible and adaptable to a variety of cell-based assays. The small volumes of the drops enables the concentrations of secreted molecules to rapidly attain detectable levels. We show that single hybridoma cells in 33 pL drops secrete detectable concentrations of antibodies in only 6 h and remain fully viable. These devices hold the promise of developing microfluidic cell cytometers and cell sorters with much greater functionality, allowing assays to be performed on individual cells in their own microenvironment prior to analysis and sorting.

Graphical abstract: Drop-based microfluidic devices for encapsulation of single cells

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Publication details

The article was received on 20 Feb 2008, accepted on 24 Apr 2008 and first published on 23 May 2008


Article type: Paper
DOI: 10.1039/B802941E
Citation: Lab Chip, 2008,8, 1110-1115
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    Drop-based microfluidic devices for encapsulation of single cells

    S. Köster, F. E. Angilè, H. Duan, J. J. Agresti, A. Wintner, C. Schmitz, A. C. Rowat, C. A. Merten, D. Pisignano, A. D. Griffiths and D. A. Weitz, Lab Chip, 2008, 8, 1110
    DOI: 10.1039/B802941E

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