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Issue 7, 2008
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Concentration gradient generator using a convective–diffusive balance

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Abstract

Since biomolecular concentration gradients are an important factor in various biological phenomena, many concentration gradient generators utilizing microfluidic techniques have been devised in order to establish stable in vitro biomolecular concentration gradients, each with their own strengths. However, all the fundamental issues, simplicity in apparatus, dynamic controllability, and convection-free conditions, have not been addressed simultaneously. In this paper, we describe a novel way of establishing concentration gradients using a convective–diffusive balance in a counterflow configuration. With the help of this method, stable, reproducible concentration gradient profiles can be formed in under a minute and the shape of the profiles are easily predicted with a simple mathematical formula.

Graphical abstract: Concentration gradient generator using a convective–diffusive balance

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Publication details

The article was received on 17 Jan 2008, accepted on 23 Apr 2008 and first published on 19 May 2008


Article type: Technical Note
DOI: 10.1039/B800859K
Citation: Lab Chip, 2008,8, 1220-1222
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    Concentration gradient generator using a convective–diffusive balance

    T. Kang, J. Han and K. S. Lee, Lab Chip, 2008, 8, 1220
    DOI: 10.1039/B800859K

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