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Issue 6, 2008
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Microfluidic device for label-free measurement of platelet activation

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Abstract

In this work we demonstrate a new microfluidic method for the rapid assessment of platelet size and morphology in whole blood. The device continuously fractionates particles according to size by displacing them perpendicularly to the fluid flow direction in a micro-fabricated post array. Whole blood, labeled with the fluorescent, platelet specific, antibody PE-anti-CD41, was run through the device and the positions of fluorescent objects noted as they exited the array. From this, histograms of platelet size were created which show marked increases in size after exposure to thrombin or a temperature of 4 °C. We infer that the well known morphological changes that occur during activation are causing the observed increase in size.

Graphical abstract: Microfluidic device for label-free measurement of platelet activation

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Publication details

The article was received on 15 Jan 2008, accepted on 19 Mar 2008 and first published on 11 Apr 2008


Article type: Paper
DOI: 10.1039/B800721G
Citation: Lab Chip, 2008,8, 925-931
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    Microfluidic device for label-free measurement of platelet activation

    D. W. Inglis, K. J. Morton, J. A. Davis, Thomas. J. Zieziulewicz, D. A. Lawrence, R. H. Austin and J. C. Sturm, Lab Chip, 2008, 8, 925
    DOI: 10.1039/B800721G

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