Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber

Kwang Ho Cheong; Dong Kee Yi; Jeong-Gun Lee; Jong-Myeon Park; Min Jun Kim; Joshua B. Edel; Christopher Ko

doi:10.1039/B717382B

skip RSC | ChemSpider | Feedback

Home > Journals > Lab on a Chip > Gold nanoparticles for ...

Authors & Referees | Librarians

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Lab on a Chip

Issue 5, 2008

More about this Journal
Editorial Board
Submit an Article

Follow Journal | |

Journal Home | RSC Journals

Technical Note

Previous Article | Next Article

Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber

Kwang Ho Cheong , Dong Kee Yi , Jeong-Gun Lee , Jong-Myeon Park , Min Jun Kim , Joshua B. Edel and Christopher Ko

Lab Chip, 2008, 8, 810-813

DOI: 10.1039/B717382B
Received 09 Nov 2007, Accepted 25 Feb 2008
First published on the web 11 Mar 2008

Please wait while Download options loads

Request Permissions

Abstract
Cited by
Related Content

The optothermal properties of nanoparticles are of interest for biosensors and highly sensitive biochip applications. In this respect, the longitudinal resonance of Au nanorods was used to transform near infrared energy into thermal energy in a microfluidic chip. The resulting heat generated effectively caused pathogen lysis. Consequently the DNA was extracted out of the cell body and transferred to a PCR system. This resulted in the successful demonstration of a one step real-time PCR system for pathogen detection without removal or changing of reagents.

Abstract from Analytical Abstracts 2008

Supplementary Info

UV-Vis spectra for the nanoparticles, laser power effects on the optothermal property of Au rods,and experimental details.
PDF (237K)

Articles By

Kwang Ho Cheong
Dong Kee Yi
Jeong-Gun Lee
Jong-Myeon Park
Min Jun Kim
Joshua B. Edel
Christopher Ko

Terms & Conditions|Privacy |Accessibility|ACAP Enabled

Lab on a Chip

Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber

Log in (Free Access)

Log in (Subscriber Access)

Supplementary Info

Articles By