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Issue 8, 2008
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Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

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Abstract

We have applied the microfluidic cell separation method of dielectrophoretic field-flow fractionation (DEP-FFF) to the enrichment of a putative stem cell population from an enzyme-digested adipose tissue derived cell suspension. A DEP-FFF separator device was constructed using a novel microfluidic-microelectronic hybrid flex-circuit fabrication approach that is scaleable and anticipates future low-cost volume manufacturing. We report the separation of a nucleated cell fraction from cell debris and the bulk of the erythrocyte population, with the relatively rare (<2% starting concentration) NG2-positive cell population (pericytes and/or putative progenitor cells) being enriched up to 14-fold. This work demonstrates a potential clinical application for DEP-FFF and further establishes the utility of the method for achieving label-free fractionation of cell subpopulations.

Graphical abstract: Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

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Publication details

The article was received on 05 Nov 2007, accepted on 24 Apr 2008 and first published on 28 May 2008


Article type: Technical Note
DOI: 10.1039/B717043B
Citation: Lab Chip, 2008,8, 1386-1393
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    Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation

    J. Vykoukal, D. M. Vykoukal, S. Freyberg, E. U. Alt and P. R. C. Gascoyne, Lab Chip, 2008, 8, 1386
    DOI: 10.1039/B717043B

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