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Issue 3, 2008
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A serial dilution microfluidic device using a ladder network generating logarithmic or linear concentrations

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Abstract

In this paper, we propose a serial dilution microfluidic chip which is able to generate logarithmic or linear step-wise concentrations. These concentrations were generated via adjustments in the flow rate of two converging fluids at the channel junctions of the ladder network. The desired dilution ratios are almost independent of the flow rate or diffusion length of molecules, as the dilution device is influenced only by the ratio of volumetric flow rates. Given a set of necessary dilution ratios, whether linear or logarithmic, a serial dilution chip can be constructed via the modification of a microfluidic resistance network. The design principle was suggested and both the logarithmic and linear dilution chips were fabricated in order to verify their performance in accordance with the fluorescence intensity. The diluted concentrations of a fluorescein solution in the microfluidic device evidenced relatively high linearity, and the cytotoxicity test of MCF-7 breast cancer cellsvia the logarithmic dilution chip was generally consistent with the results generated with manual dilution.

Graphical abstract: A serial dilution microfluidic device using a ladder network generating logarithmic or linear concentrations

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Publication details

The article was received on 20 Sep 2007, accepted on 02 Jan 2008 and first published on 01 Feb 2008


Article type: Paper
DOI: 10.1039/B714536E
Citation: Lab Chip, 2008,8, 473-479
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    A serial dilution microfluidic device using a ladder network generating logarithmic or linear concentrations

    C. Kim, K. Lee, J. H. Kim, K. S. Shin, K. Lee, T. S. Kim and J. Y. Kang, Lab Chip, 2008, 8, 473
    DOI: 10.1039/B714536E

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