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Issue 2, 2008
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Model-controlled hydrodynamic focusing to generate multiple overlapping gradients of surface-immobilized proteins in microfluidic devices

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Abstract

Historically, it has been difficult to generate accurate and reproducible protein gradients for studies of interactions between cells and extracellular matrix. Here we demonstrate a method for rapid patterning of protein gradients using computer-driven hydrodynamic focusing in a simple microfluidic device. In contrast to published work, we are moving the complexity of gradient creation from the microfluidic hardware to dynamic computer control. Using our method, switching from one gradient profile to another requires only a few hours to devise a new control file, not days or weeks to design and build a new microfluidic device. Fitting existing protein deposition models to our data, we can extract key parameters needed for controlling protein deposition. Several protein deposition models were evaluated under microfluidic flow conditions. A mathematical model for our deposition method allows us to determine the parameters for a protein adsorption model and then predict the final shape of the surface density gradient. Simple and non-monotonic single and multi-protein gradient profiles were designed and deposited using the same device.

Graphical abstract: Model-controlled hydrodynamic focusing to generate multiple overlapping gradients of surface-immobilized proteins in microfluidic devices

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Publication details

The article was received on 26 Jun 2007, accepted on 30 Nov 2007 and first published on 21 Dec 2007


Article type: Paper
DOI: 10.1039/B716203K
Citation: Lab Chip, 2008,8, 238-244
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    Model-controlled hydrodynamic focusing to generate multiple overlapping gradients of surface-immobilized proteins in microfluidic devices

    W. Georgescu, J. Jourquin, L. Estrada, A. R. A. Anderson, V. Quaranta and J. P. Wikswo, Lab Chip, 2008, 8, 238
    DOI: 10.1039/B716203K

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