Jump to main content
Jump to site search

Issue 5, 2008
Previous Article Next Article

Separation and identification of the selenium-sulfur amino acid S-(methylseleno)cysteine in intestinal epithelial cell homogenates by LC-ICP-MS and LC-ESI-MS after incubation with methylseleninic acid

Author affiliations

Abstract

The metabolism of methylseleninic acid, MeSeA in the gastro-intestinal tract was studied by incubation of MeSeA with homogenized intestinal epithelial cells from pigs. The major selenium-containing metabolite was identified by analysis of the supernatant of the incubated cells by LC-ICP-MS. The identity of the compound was established by LC-ESI-MS/MS after purification by preparative chromatography. The metabolite appeared to be a selenium-sulfur amino acid, S-(methylseleno)cysteine (CH3Se–S–CH2CH(NH2)COOH). The selenium-sulfur ratio was confirmed to be 1:1 by simultaneous monitoring of selenium and sulfur as the oxides by LC-ICP-MS. Analysis of a synthesized standard of S-(methylseleno)cysteine by LC-ESI-MS resulted in the same mass spectrum with the same fragmentation pattern as the isolated metabolite. The formation of this selenium compound did not require enzymatic systems but only the presence of cysteine. The presence of this selenium compound in mammalian cell models after addition of MeSeA has not previously been reported.

Graphical abstract: Separation and identification of the selenium-sulfur amino acid S-(methylseleno)cysteine in intestinal epithelial cell homogenates by LC-ICP-MS and LC-ESI-MS after incubation with methylseleninic acid

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 15 Oct 2007, accepted on 11 Feb 2008 and first published on 28 Feb 2008


Article type: Paper
DOI: 10.1039/B715899H
Citation: J. Anal. At. Spectrom., 2008,23, 727-732
  •   Request permissions

    Separation and identification of the selenium-sulfur amino acid S-(methylseleno)cysteine in intestinal epithelial cell homogenates by LC-ICP-MS and LC-ESI-MS after incubation with methylseleninic acid

    C. Gabel-Jensen, K. Lunøe, K. G. Madsen, J. Bendix, C. Cornett, S. Stürup, H. R. Hansen and B. Gammelgaard, J. Anal. At. Spectrom., 2008, 23, 727
    DOI: 10.1039/B715899H

Search articles by author