Jump to main content
Jump to site search

Volume 139, 2008
Previous Article Next Article

Non-cytotoxic polymer vesicles for rapid and efficient intracellular delivery

Author affiliations

Abstract

We have recently achieved efficient cytosolic delivery by using pH-sensitive poly(2-(methacryloyloxy)ethylphosphorylcholine)-co-poly(2-(diisopropylamino)ethylmethacrylate) (PMPCPDPA) diblock copolymers that self-assemble to form vesicles, known as polymersomes, in aqueous solution. It is particularly noteworthy that these diblock copolymers form stable polymersomes at physiological pH but rapidly dissociate below pH 6 to give molecularly-dissolved copolymer chains (unimers). These PMPCPDPA polymersomes are used to encapsulate nucleic acids for efficient intracellular delivery. Confocal laser scanning microscopy and fluorescence flow cytometry are used to quantify cellular uptake and to study the kinetics of this process. Finally, we examine how PMPCPDPA polymersomes affect the viability of primary human cells (human dermal fibroblasts (HDF)), paying particular regard to whether inflammatory responses are triggered.

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 12 Nov 2007, accepted on 09 Jan 2008 and first published on 22 Apr 2008


Article type: Paper
DOI: 10.1039/B717431D
Citation: Faraday Discuss., 2008,139, 143-159
  •   Request permissions

    Non-cytotoxic polymer vesicles for rapid and efficient intracellular delivery

    H. Lomas, M. Massignani, K. A. Abdullah, I. Canton, C. Lo Presti, S. MacNeil, J. Du, A. Blanazs, J. Madsen, S. P. Armes, A. L. Lewis and G. Battaglia, Faraday Discuss., 2008, 139, 143
    DOI: 10.1039/B717431D

Search articles by author