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Volume 139, 2008
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Non-cytotoxic polymer vesicles for rapid and efficient intracellular delivery

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We have recently achieved efficient cytosolic delivery by using pH-sensitive poly(2-(methacryloyloxy)ethylphosphorylcholine)-co-poly(2-(diisopropylamino)ethylmethacrylate) (PMPCPDPA) diblock copolymers that self-assemble to form vesicles, known as polymersomes, in aqueous solution. It is particularly noteworthy that these diblock copolymers form stable polymersomes at physiological pH but rapidly dissociate below pH 6 to give molecularly-dissolved copolymer chains (unimers). These PMPCPDPA polymersomes are used to encapsulate nucleic acids for efficient intracellular delivery. Confocal laser scanning microscopy and fluorescence flow cytometry are used to quantify cellular uptake and to study the kinetics of this process. Finally, we examine how PMPCPDPA polymersomes affect the viability of primary human cells (human dermal fibroblasts (HDF)), paying particular regard to whether inflammatory responses are triggered.

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Publication details

The article was received on 12 Nov 2007, accepted on 09 Jan 2008 and first published on 22 Apr 2008

Article type: Paper
DOI: 10.1039/B717431D
Citation: Faraday Discuss., 2008,139, 143-159
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    Non-cytotoxic polymer vesicles for rapid and efficient intracellular delivery

    H. Lomas, M. Massignani, K. A. Abdullah, I. Canton, C. Lo Presti, S. MacNeil, J. Du, A. Blanazs, J. Madsen, S. P. Armes, A. L. Lewis and G. Battaglia, Faraday Discuss., 2008, 139, 143
    DOI: 10.1039/B717431D

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