Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription–translation of green fluorescent protein as a report gate

Takahiko Nojima; Takatoki Yamamoto; Hiroshi Kimura; Teruo Fujii

doi:10.1039/B807039C

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Chemical Communications

Issue 32, 2008

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Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription–translation of green fluorescent protein as a report gate

Takahiko Nojima , Takatoki Yamamoto , Hiroshi Kimura and Teruo Fujii

Chem. Commun., 2008, 3771-3773

DOI: 10.1039/B807039C
Received 25 Apr 2008, Accepted 04 Jun 2008
First published on the web 17 Jun 2008

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Polymerase chain reaction-based biochemical logic gates were designed for AND, OR, NOT, and AND–NOT operations, whose output signal is reported by coupled cell-free transcription–translation of green fluorescent protein.

Supplementary Info

Construction of the logic gate DNA templates, PCR conditions in the logic gates
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Chemical Communications

Polymerase chain reaction-based biochemical logic gate coupled with cell-free transcription–translation of green fluorescent protein as a report gate

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