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Issue 1, 2008
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Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences

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Abstract

We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assayZ-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 × 10−11 M concentration.

Graphical abstract: Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences

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Publication details

The article was received on 06 Aug 2007, accepted on 06 Sep 2007 and first published on 21 Sep 2007


Article type: Paper
DOI: 10.1039/B711850C
Citation: Analyst, 2008,133, 52-57
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    Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences

    Y. Uludağ, X. Li, H. Coleman, S. Efstathiou and M. A. Cooper, Analyst, 2008, 133, 52
    DOI: 10.1039/B711850C

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