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Issue 12, 2007
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Light fractionation does not enhance the efficacy of methyl 5-aminolevulinate mediated photodynamic therapy in normal mouse skin

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Abstract

Previous work demonstrated that fractionated illumination using two fractions separated by a dark interval of 2 h, significantly enhanced the clinical efficacy of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA). Considering the increasing clinical use of methyl 5-aminolevulinate (MAL) and the expected gain in efficacy by light fractionation we have investigated the response to MAL-PDT using a single and a two-fold illumination scheme and compared that with ALA-PDT. Our results show that fractionated illumination does not enhance the efficacy of PDT using MAL as it does using ALA despite the comparable fluorescence intensities at the end of the first light fraction and at the start of the second light fraction. Only the initial rate of photobleaching was slightly greater during ALA-PDT although the difference was small. Previously we hypothesized that cells surviving the first fraction are more susceptible to the second fraction. Since this is not true for MAL-PDT our data suggest that the distribution of MAL and ALA in tissues, and therefore the site of PDT induced damage, is an important parameter in the mechanism underlying the 2-fold illumination scheme.

Graphical abstract: Light fractionation does not enhance the efficacy of methyl 5-aminolevulinate mediated photodynamic therapy in normal mouse skin

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Publication details

The article was received on 04 Jun 2007, accepted on 27 Jul 2007 and first published on 28 Aug 2007


Article type: Paper
DOI: 10.1039/B708340H
Citation: Photochem. Photobiol. Sci., 2007,6, 1325-1331
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    Light fractionation does not enhance the efficacy of methyl 5-aminolevulinate mediated photodynamic therapy in normal mouse skin

    H. S. de Bruijn, E. R. M. de Haas, K. M. Hebeda, A. van der Ploeg - van den Heuvel, H. J. C. M. Sterenborg, H. A. M. Neumann and D. J. Robinson, Photochem. Photobiol. Sci., 2007, 6, 1325
    DOI: 10.1039/B708340H

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