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Issue 9, 2007
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Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers

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Abstract

Three porphyrin systems have been characterised for use in two-photon fluorescence imaging of biological samples. We have determined the two-photon absorption cross sections (σ2) of the di-cation, free-base and metallated forms of hematoporphyrin derivative (HpD), hematoporphyrin IX (Hp9) and a boronated protoporphyrin (BOPP) using the open-aperture Z-scan and the two-photon induced fluorescence (TPIF) techniques at an excitation wavelength of 800 nm. The insertion of either protons or a metal ion into the macrocycle is shown not to significantly influence the σ2 of the porphyrins. Two-photon time-resolved fluorescence images of C6 glioma cells transfected with a free-base form of the BOPP have been obtained as a function of the porphyrin concentration. These studies reveal a maximum useful porphyrin concentration for fluorescence imaging purposes of approximately 30 µg mL−1.

Graphical abstract: Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers

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Publication details

The article was received on 03 Apr 2007, accepted on 05 Jul 2007 and first published on 19 Jul 2007


Article type: Paper
DOI: 10.1039/B705101H
Citation: Photochem. Photobiol. Sci., 2007,6, 1019-1026
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    Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers

    S. Mathai, D. K. Bird, S. S. Stylli, T. A. Smith and K. P. Ghiggino, Photochem. Photobiol. Sci., 2007, 6, 1019
    DOI: 10.1039/B705101H

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