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Issue 9, 2007
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Ultrafast light harvesting dynamics in the cryptophyte phycocyanin 645

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Steady-state and femtosecond time-resolved optical methods have been used to study spectroscopic features and energy transfer dynamics in the soluble antenna protein phycocyanin 645 (PC645), isolated from a unicellular cryptophyte Chroomonas CCMP270. Absorption, emission and polarization measurements as well as one-colour pump–probe traces are reported in combination with complementary quantum chemical calculations of electronic transitions of the bilins. Estimation of bilin spectral positions and energy transfer rates aids in the development of a model for light harvesting by PC645. At higher photon energies light is absorbed by the centrally located dimer (DBV, β50/β61) and the excitation is subsequently funneled through a complex interference of pathways to four peripheral pigments (MBV α19, PCB β158). Those chromophores transfer the excitation energy to the red-most bilins (PCB β82). We suggest that the final resonance energy transfer step occurs between the PCB 82 bilins on a timescale estimated to be ∼15 ps. Such a rapid final energy transfer step cannot be rationalized by calculations that combine experimental parameters and quantum chemical calculations, which predict the energy transfer time to be 40 ps.

Graphical abstract: Ultrafast light harvesting dynamics in the cryptophyte phycocyanin 645

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Publication details

The article was received on 03 Apr 2007, accepted on 04 Jul 2007 and first published on 12 Jul 2007

Article type: Paper
DOI: 10.1039/B704962E
Citation: Photochem. Photobiol. Sci., 2007,6, 964-975
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    Ultrafast light harvesting dynamics in the cryptophyte phycocyanin 645

    T. Mirkovic, A. B. Doust, J. Kim, K. E. Wilk, C. Curutchet, B. Mennucci, R. Cammi, P. M. G. Curmi and G. D. Scholes, Photochem. Photobiol. Sci., 2007, 6, 964
    DOI: 10.1039/B704962E

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