Issue 1, 2007
From the journal:

Photochemical & Photobiological Sciences

ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starchextraction

Mateus Borba Cardoso,a   Dimitrios Samios,a   Nádya Pesce da Silveira,*a   Fabiano Severo Rodembusch*b  and  Valter Stefanib  

* Corresponding authors

a Universidade Federal do Rio Grande do Sul-Instituto de Química, Laboratório de Instrumentação e Dinâmica Molecular, Av. Bento Gonçalves, 9500. CP 15003 CEP 91501-970, Porto Alegre-RS, Brazil
E-mail: nadya@iq.ufrgs.br
Fax: +55 51 3316 7304
Tel: +55 51 3316 6291

b Laboratório de Novos Materiais Orgânicos, Av. Bento Gonçalves, 9500. CP 15003 CEP 91501-970, Porto Alegre-RS, Brazil
E-mail: rodembusch@iq.ufrgs.br
Tel: +55 51 3316 6285

Abstract

The 2-(4′-isothiocyanate-2′-hydroxyphenyl)benzoxazole dye was successfully applied as label of rice proteins during the alkaline extraction of starch. Direct fluorescence measurements were used to observe the presence of proteins labelled in different steps of rice starch extraction. The results were compared to those obtained with the well-known biuret colorimetric test. Whereas the colorimetric test indicates the absence of protein after the third extraction step, the fluorescence emission of the conjugate could be observed in all extraction steps. The separation of different rice proteins could also be observed.

Graphical abstract: ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction

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Article information

DOI
https://doi.org/10.1039/B610999C
Article type
Paper
Submitted
01 Aug 2006
Accepted
06 Oct 2006
First published
02 Nov 2006

Photochem. Photobiol. Sci., 2007,6, 99-102

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ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction

M. B. Cardoso, D. Samios, N. P. da Silveira, F. S. Rodembusch and V. Stefani, Photochem. Photobiol. Sci., 2007, 6, 99 DOI: 10.1039/B610999C

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