Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy

Ingo Gregor; Jörg Enderlein

doi:10.1039/B610310C

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Photochemical & Photobiological Sciences

Issue 1, 2007

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Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy

Ingo Gregor and Jörg Enderlein

Photochem. Photobiol. Sci., 2007, 6, 13-18

DOI: 10.1039/B610310C
Received 18 Jul 2006, Accepted 26 Oct 2006
First published on the web 14 Nov 2006
This article is part of the collection: Time-resolved methods in biophysics series

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We present a thorough introduction into the recently developed fluorescence lifetime correlation spectroscopy (FLCS). The theoretical basis of FLCS is explained, and the method is applied to the study of a dynamic transition between two fluorescence lifetime states in a dye–protein complex.

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Photochemical & Photobiological Sciences

Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy

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