Sequence specific interstrand photocrosslinking for effective SNP typing

Takehiro Ami; Katsuya Ito; Yoshinaga Yoshimura; Kenzo Fujimoto

doi:10.1039/B708264A

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Organic & Biomolecular Chemistry

Issue 16, 2007

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Sequence specific interstrand photocrosslinking for effective SNP typing

Takehiro Ami , Katsuya Ito , Yoshinaga Yoshimura and Kenzo Fujimoto

Org. Biomol. Chem., 2007, 5, 2583-2586

DOI: 10.1039/B708264A
Received 31 May 2007, Accepted 18 Jun 2007
First published on the web 02 Jul 2007

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We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p-carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide specificity as high as 10³-fold and more, and can be used in the diagnostic detection of DNA sequences.

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Organic & Biomolecular Chemistry

Sequence specific interstrand photocrosslinking for effective SNP typing

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