Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes

Zoya Cheglakov; Yossi Weizmann; Bernhard Basnar; Itamar Willner

doi:10.1039/B615450F

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Organic & Biomolecular Chemistry

Issue 2, 2007

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Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes

Zoya Cheglakov , Yossi Weizmann , Bernhard Basnar and Itamar Willner

Org. Biomol. Chem., 2007, 5, 223-225

DOI: 10.1039/B615450F
Received 24 Oct 2006, Accepted 20 Nov 2006
First published on the web 06 Dec 2006

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Abstract
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Circular DNA is used as a template for the amplified detection of M13 phage ssDNA by a rolling circle amplification (RCA) process that synthesizes DNAzyme chains, thus enabling the colorimetric or chemiluminescent detection of the analyte.

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Materials, circular DNA template preparation and the RCA assay
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Organic & Biomolecular Chemistry

Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes

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