Issue 22, 2007
From the journal:

Organic & Biomolecular Chemistry

Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity†

Martin D. Witte,a   Carlos V. Descals,a   Sebastiaan V. P. de Lavoir,a   Bogdan I. Florea,a   Gijsbert A. van der Marel*a  and  Herman S. Overkleeft*a  

* Corresponding authors

a Bioorganic Synthesis, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, The Netherlands
E-mail: marel_g@chem.leidenuniv.nl, h.s.overkleeft@chem.leidenuniv.nl
Fax: 31 71 527 4307
Tel: 31 71 527 4342

Abstract

In this paper the development of a fluorescent activity based probe, Bodipy-VAD-Fmk, for visualization of yeast peptide N-glycanase is described. The activity based probe is used to assess the efficacy of known and new chitobiose-based electrophilic traps to bind yeast peptide N-glycanase.

Graphical abstract: Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity

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Article information

DOI
https://doi.org/10.1039/B711531H
Article type
Paper
Submitted
27 Jul 2007
Accepted
13 Aug 2007
First published
29 Aug 2007

Org. Biomol. Chem., 2007,5, 3690-3697

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Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity

M. D. Witte, C. V. Descals, S. V. P. de Lavoir, B. I. Florea, G. A. van der Marel and H. S. Overkleeft, Org. Biomol. Chem., 2007, 5, 3690 DOI: 10.1039/B711531H

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