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Issue 12, 2007
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Water-soluble carbosilane dendrimers protect phosphorothioate oligonucleotides from binding to serum proteins

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Abstract

Treatment of dendriplexes formed between water-soluble carbosilane dendrimers and phosphorothioate oligodeoxynucleotides (ODN) with the anionic detergentsodium dodecyl sulfate disrupted the complexes indicating that the nature of the union in such dendriplexes is merely electrostatic. However, dendriplexes were not dissociated by serum proteins like bovine or human serum albumins, as assessed by gel electrophoresis and fluorescence experiments. This would imply a dendrimer-mediated protective effect able to prevent ODN interactions with serum proteins and additionally could translate into a reduction of the ODN doses needed to achieve the biological effects. The employment of carbosilane dendrimers as carriers may solve the problem of ODN kidnapping by plasmatic proteins as a key drawback for therapeutics involving ODNs. As examples, transfection processes on normal primary peripheral blood cells and diagnosis of HIV infection in the presence of serum have been assayed.

Graphical abstract: Water-soluble carbosilane dendrimers protect phosphorothioate oligonucleotides from binding to serum proteins

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Publication details

The article was received on 16 Mar 2007, accepted on 27 Apr 2007 and first published on 14 May 2007


Article type: Paper
DOI: 10.1039/B703989A
Citation: Org. Biomol. Chem., 2007,5, 1886-1893
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    Water-soluble carbosilane dendrimers protect phosphorothioate oligonucleotides from binding to serum proteins

    L. Chonco, J. F. Bermejo-Martín, P. Ortega, D. Shcharbin, E. Pedziwiatr, B. Klajnert, F. Javier de la Mata, R. Eritja, R. Gómez, M. Bryszewska and M. xmlns="http://www.rsc.org/schema/rscart38"> <. Angeles Muñoz-Fernandez, Org. Biomol. Chem., 2007, 5, 1886
    DOI: 10.1039/B703989A

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