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Issue 11, 2007
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Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution

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Abstract

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid progress is being made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. This review outlines developments in far-field fluorescence methods which enable ultrahigh resolution imaging and application of these techniques to biology. Future possible trends and directions in far-field fluorescence imaging with ultrahigh resolution are also outlined.

Graphical abstract: Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution

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Publication details

The article was received on 11 Apr 2007, accepted on 16 Aug 2007 and first published on 11 Sep 2007


Article type: Review Article
DOI: 10.1039/B705460B
Citation: Mol. BioSyst., 2007,3, 781-793
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    Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution

    J. H. Rice, Mol. BioSyst., 2007, 3, 781
    DOI: 10.1039/B705460B

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