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Issue 12, 2007
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Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels

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Abstract

The interactions between endothelial cells and the underlying extracellular matrix regulate adhesion and cellular responses to microenvironmental stimuli, including flow-induced shear stress. In this study, we investigated the adhesion properties of primary porcine aortic endothelial cells (PAECs) and valve endothelial cells (PAVECs) in a microfluidic network. Taking advantage of the parallel arrangement of the microchannels, we compared adhesion of PAECs and PAVECs to fibronectin and type I collagen, two prominent extracellular matrix proteins, over a broad range of concentrations. Cell spreading was measured morphologically, based on cytoplasmic staining with a vital dye, while adhesion strength was characterized by the number of cells attached after application of shear stresses of 11, 110, and 220 dyn cm−2. Results showed that PAVECs were more well spread on fibronectin than on type I collagen (P < 0.0001), particularly for coating concentrations of 100, 200, and 500 µg mL−1. PAVECs also withstood shear significantly better on fibronectin than on collagen for 500 µg mL−1. PAECs were more well spread on collagen compared to PAVECs (P < 0.0001), but did not have significantly better adhesion strength. These results demonstrate that cell adhesion is both cell-type and matrix dependent. Furthermore, they reveal important phenotypic differences between vascular and valvular endothelium, with implications for endothelial mechanobiology and the design of microdevices and engineered tissues.

Graphical abstract: Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels

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Publication details

The article was received on 14 Aug 2007, accepted on 31 Aug 2007 and first published on 14 Sep 2007


Article type: Paper
DOI: 10.1039/B712486D
Citation: Lab Chip, 2007,7, 1759-1766
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    Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels

    E. W. K. Young, A. R. Wheeler and C. A. Simmons, Lab Chip, 2007, 7, 1759
    DOI: 10.1039/B712486D

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