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Issue 12, 2007
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Integrated microfluidic cell culture and lysis on a chip

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Abstract

We present an integrated microfluidic cell culture and lysis platform for automated cell analysis that improves on systems which require multiple reagents and manual procedures. Through the combination of previous technologies developed in our lab (namely, on-chip cell culture and electrochemical cell lysis) we have designed, fabricated, and characterized an integrated microfluidic platform capable of culturing HeLa, MCF-7, Jurkat, and CHO-K1 cells for up to five days and subsequently lysing the cells without the need to add lysing reagents. On-demand lysis was accomplished by local hydroxide ion generation within microfluidic chambers, releasing both proteinacious (GFP) and genetic (Hoescht-stained DNA) material. Sample proteins exposed to the electrochemical lysis conditions were immunodetectable (p53) and their enzymatic activity (HRP) was investigated.

Graphical abstract: Integrated microfluidic cell culture and lysis on a chip

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Publication details

The article was received on 06 Aug 2007, accepted on 27 Sep 2007, published on 19 Oct 2007 and first published online on 19 Oct 2007


Article type: Paper
DOI: 10.1039/B711874K
Citation: Lab Chip, 2007,7, 1689-1695
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    Integrated microfluidic cell culture and lysis on a chip

    J. T. Nevill, R. Cooper, M. Dueck, D. N. Breslauer and L. P. Lee, Lab Chip, 2007, 7, 1689
    DOI: 10.1039/B711874K

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