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Issue 12, 2007
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Cell-based high content screening using an integrated microfluidic device

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Abstract

High content screening (HCS) has quickly established itself as a core technique in the early stage of drug discovery for secondary compound screening. It allows several independent cellular parameters to be measured in a single cell or populations of cells in a single assay. In this work, we describe high content screening for the multiparametric measurement of cellular responses in human liver carcinoma (HepG2) cells using an integrated microfluidic device. This device consists of multiple drug gradient generators and parallel cell culture chambers, in which the processes of liquid dilution and diffusion, micro-scale cell culture, cell stimulation and cell labeling can be integrated into a single device. The simple assay provides multiparametric measurements of plasma membrane permeability, nuclear size, mitochondrial transmembrane potential and intracellular redox states in anti-cancer drug-induced apoptosis of HepG2 cells. The established platform is able to rapidly extract the maximum of information from tumor cells in response to several drugs varying in concentration, with minimal sample and less time, which is very useful for basic biomedical research and cancer treatment.

Graphical abstract: Cell-based high content screening using an integrated microfluidic device

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Publication details

The article was received on 27 Jul 2007, accepted on 07 Sep 2007 and first published on 08 Oct 2007


Article type: Paper
DOI: 10.1039/B711513J
Citation: Lab Chip, 2007,7, 1696-1704
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    Cell-based high content screening using an integrated microfluidic device

    N. Ye, J. Qin, W. Shi, X. Liu and B. Lin, Lab Chip, 2007, 7, 1696
    DOI: 10.1039/B711513J

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