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Issue 12, 2007
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Polar stimulation and constrained cell migration in microfluidic channels

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Abstract

Asymmetrical delivery of stimuli to moving cells for perturbing spatially-heterogeneous intracellular signaling is an experimental challenge not adequately met by existing technologies. Here, we report a robust microfluidic platform allowing localized treatment of the front and/or back of moving cells which crawl through narrow channels that they completely occlude. The enabling technical element for this study is a novel design for precise, passive balancing of flow inside the microfluidic device by contacting two fluid streams before splitting them again. The microchannels constrain cell morphology and induce qualitative and quantitative changes in neutrophil chemotaxis that mimic cells crawling through tissues.

Graphical abstract: Polar stimulation and constrained cell migration in microfluidic channels

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Publication details

The article was received on 10 Jul 2007, accepted on 17 Aug 2007 and first published on 04 Sep 2007


Article type: Paper
DOI: 10.1039/B710524J
Citation: Lab Chip, 2007,7, 1783-1790
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    Polar stimulation and constrained cell migration in microfluidic channels

    D. Irimia, G. Charras, N. Agrawal, T. Mitchison and M. Toner, Lab Chip, 2007, 7, 1783
    DOI: 10.1039/B710524J

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