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Issue 11, 2007
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Self-assembled cellular microarrays patterned using DNA barcodes

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Abstract

The successful integration of living cells into synthetic devices requires precise control over cell patterning. Here we describe a versatile platform that can accomplish this goal through DNA hybridization. Living cells functionalized with exogenous cell-surface DNA strands bind to cognate sequences of DNA printed on glass slides. Attachment via these “cell-adhesion barcodes” is rapid and specific, with close-packed arrays of cells forming within minutes. The biophysical properties of the system are characterized, and the technique is used to form complex cellular patterns with single-cell line widths and self-assembled cellular microarrays. Key advantages of DNA-directed cell binding include the ability to immobilize both adherent and non-adherent cells, to capture cells selectively from a mixed population, to tune the binding properties of the cells, and to reuse substrates prepared with widely available DNA printing technologies.

Graphical abstract: Self-assembled cellular microarrays patterned using DNA barcodes

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Publication details

The article was received on 07 Jun 2007, accepted on 28 Aug 2007 and first published on 19 Sep 2007


Article type: Paper
DOI: 10.1039/B708666K
Citation: Lab Chip, 2007,7, 1442-1448
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    Self-assembled cellular microarrays patterned using DNA barcodes

    E. S. Douglas, R. A. Chandra, C. R. Bertozzi, R. A. Mathies and M. B. Francis, Lab Chip, 2007, 7, 1442
    DOI: 10.1039/B708666K

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