Jump to main content
Jump to site search

Issue 11, 2007
Previous Article Next Article

Mass spectrometric imaging of peptide release from neuronal cells within microfluidic devices

Author affiliations

Abstract

Microfluidic devices are well suited for manipulating and measuring mass limited samples. Here we adapt a microfluidic device containing functionalized surfaces to chemically stimulate a small number of neurons (down to a single neuron), collect the release of neuropeptides, and characterize them using mass spectrometry. As only a small fraction of the peptides present in a neuron are released with physiologically relevant stimulations, the amount of material available for measurement is small, thereby requiring minimal sample loss and high-sensitivity detection. Although a number of detection schemes are used with microfluidic devices, mass spectrometric detection is used here because of its high information content, allowing the characterization of the released peptide complement. Rather than using an on-line approach, off-line analysis is used; after collection of the peptides onto a surface, mass spectrometric imaging interrogates that surface to determine the peptides released from the cell. The overall utility of this scheme is demonstrated using several device formats with measurement of neuropeptides released from Aplysia californica bag cell neurons.

Graphical abstract: Mass spectrometric imaging of peptide release from neuronal cells within microfluidic devices

Back to tab navigation

Publication details

The article was received on 09 May 2007, accepted on 25 Jul 2007 and first published on 15 Aug 2007


Article type: Paper
DOI: 10.1039/B706940E
Citation: Lab Chip, 2007,7, 1454-1460
  •   Request permissions

    Mass spectrometric imaging of peptide release from neuronal cells within microfluidic devices

    K. Jo, M. L. Heien, L. B. Thompson, M. Zhong, R. G. Nuzzo and J. V. Sweedler, Lab Chip, 2007, 7, 1454
    DOI: 10.1039/B706940E

Search articles by author

Spotlight

Advertisements