Cell encapsulating droplet vitrification

Utkan Demirci; Grace Montesano

doi:10.1039/B705809H

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Lab on a Chip

Issue 11, 2007

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Cell encapsulating droplet vitrification

Utkan Demirci and Grace Montesano

Lab Chip, 2007, 7, 1428-1433

DOI: 10.1039/B705809H
Received 17 Apr 2007, Accepted 25 Jul 2007
First published on the web 09 Aug 2007

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The capability to encapsulate single cells in droplets while retaining high cell viability (>90%) has great impact on tissue engineering, high-throughput screening, as well as clinical diagnostics and therapeutics. We demonstrate a novel method to vitrify a small number of cells using cell-encapsulating droplets. The method allows vitrification at low cryoprotectant concentration (1.5 M propanediol and 0.5 M trehalose), similar to that used in slow freezing protocols. The method was successfully applied to five different mammalian cell types: AML-12 hepatocytes, NIH-3T3 fibroblasts, HL-1 cardiomyocytes, mouse embryonic stem cells, and RAJI cells.

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