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Issue 9, 2007
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Controlled delivery of proteins into bilayer lipid membranes on chip

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Abstract

The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.

Graphical abstract: Controlled delivery of proteins into bilayer lipid membranes on chip

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Publication details

The article was received on 13 Mar 2007, accepted on 08 Jun 2007 and first published on 27 Jun 2007


Article type: Paper
DOI: 10.1039/B703818F
Citation: Lab Chip, 2007,7, 1176-1183
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    Controlled delivery of proteins into bilayer lipid membranes on chip

    M. Zagnoni, M. E. Sandison, P. Marius, A. G. Lee and H. Morgan, Lab Chip, 2007, 7, 1176
    DOI: 10.1039/B703818F

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