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Issue 4, 2007
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Active 3-D microscaffold system with fluid perfusion for culturing in vitro neuronal networks

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Abstract

This work demonstrated the design, fabrication, packaging, and characterization of an active microscaffold system with fluid perfusion/nutrient delivery functionalities for culturing in vitro neuronal networks from dissociated hippocampal rat pup neurons. The active microscaffold consisted of an 8 × 8 array of hollow, microfabricated, SU-8 towers (1.0 mm or 1.5 mm in height), with integrated, horizontal, SU-8 cross-members that connect adjacent towers, thus forming a 3-D grid that is conducive to branching, growth, and increased network formation of dissociated hippocampal neurons. Each microtower in the microscaffold system contained a hollow channel and multiple fluid ports for media delivery and perfusion of nutrients to the in vitro neuronal network growing within the microscaffold system. Additionally, there were two exposed Au electrodes on the outer wall of each microtower at varying heights (with insulated leads running within the microtower walls), which will later allow for integration of electrical stimulation/recording functionalities into the active microscaffold system. However, characterization of the stimulation/recording electrodes was not included in the scope of this paper. Design, fabrication, fluid packaging, and characterization of the active microscaffold system were performed. Furthermore, use of the active microscaffold system was demonstrated by culturing primary hippocampal embryonic rat pup neurons, and characterizing cell viability within the microscaffold system.

Graphical abstract: Active 3-D microscaffold system with fluid perfusion for culturing in vitro neuronal networks

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Publication details

The article was received on 30 Aug 2006, accepted on 07 Feb 2007 and first published on 01 Mar 2007


Article type: Paper
DOI: 10.1039/B700795G
Citation: Lab Chip, 2007,7, 475-482
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    Active 3-D microscaffold system with fluid perfusion for culturing in vitro neuronal networks

    L. Rowe, M. Almasri, K. Lee, N. Fogleman, G. J. Brewer, Y. Nam, B. C. Wheeler, J. Vukasinovic, A. Glezer and A. B. Frazier, Lab Chip, 2007, 7, 475
    DOI: 10.1039/B700795G

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