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Issue 3, 2007
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Integrated microelectronic device for label-free nucleic acid amplification and detection

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Abstract

We present an integrated microelectronic device for amplification and label-free detection of nucleic acids. Amplification by polymerase chain reaction (PCR) is achieved with on-chip metal resistive heaters, temperature sensors, and microfluidic valves. We demonstrate a rapid thermocycling with rates of up to 50 °C s−1 and a PCR product yield equivalent to that of a bench-top system. Amplicons within the PCR product are detected by their intrinsic charge with a silicon field-effect sensor. Similar to existing optical approaches with intercalators such as SYBR Green, our sensing approach can directly detect standard double-stranded PCR product, while in contrast, our sensor does not require labeling reagents. By combining amplification and detection on the same device, we show that the presence or absence of a particular DNA sequence can be determined by converting the analog surface potential output of the field-effect sensor to a simple digital true/false readout.

Graphical abstract: Integrated microelectronic device for label-free nucleic acid amplification and detection

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Publication details

The article was received on 22 Nov 2006, accepted on 18 Jan 2007 and first published on 08 Feb 2007


Article type: Paper
DOI: 10.1039/B617082J
Citation: Lab Chip, 2007,7, 347-354
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    Integrated microelectronic device for label-free nucleic acid amplification and detection

    C. Johnson Hou, M. Godin, K. Payer, R. Chakrabarti and S. R. Manalis, Lab Chip, 2007, 7, 347
    DOI: 10.1039/B617082J

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