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Issue 4, 2007
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A feasible approach to all-electronic digital labeling and readout for cell identification

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Abstract

We present two critical innovations that enable a unique, purely electronic approach to microfluidic whole-cell analysis, focusing on the problem of cell identification and sorting. We used fully-scalable lithographic techniques to microfabricate digital barcodes, providing a means for low-cost, large volume production. We have demonstrated molecular functionalization of the barcodes, using biotin–streptavidin, as well as human CD4 antibody, and we have successfully linked the barcodes to polystyrene beads using the biotin–streptavidin complex. This functionalization allows unique barcodes to be attached to specific cell types, based on phenotype. We have also implemented an electronic barcode readout scheme, using a radio frequency microsensor integrated in an elastomeric microfluidic channel, that can read individual barcodes at rates in excess of 1000 labels s−1. The barcodes are biologically compatible, and coupled with the electronic sensing technology, provide a route to compact, inexpensive, disposable cell identification, sorting and purification.

Graphical abstract: A feasible approach to all-electronic digital labeling and readout for cell identification

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Publication details

The article was received on 10 Nov 2006, accepted on 07 Feb 2007 and first published on 01 Mar 2007


Article type: Paper
DOI: 10.1039/B616442K
Citation: Lab Chip, 2007,7, 469-474
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    A feasible approach to all-electronic digital labeling and readout for cell identification

    D. K. Wood, G. B. Braun, J.-L. Fraikin, L. J. Swenson, N. O. Reich and A. N. Cleland, Lab Chip, 2007, 7, 469
    DOI: 10.1039/B616442K

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