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Issue 5, 2007
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One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device

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Abstract

We report a fully integrated, pathogen-specific DNA extraction device utilizing centrifugal microfluidics on a polymer based CD platform. By use of the innovative laser irradiated Ferrowax microvalve (LIFM) together with the rapid cell lysis method using laser irradiation on magnetic particles, we could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood on a CD. As a model study, DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) and E.coli were conducted. The total process of the plasma separation, mixing with magnetic beads conjugated with target specific antibodies, removal of plasma residual, washing and DNA extraction was finished within 12 min with only one manual step, the loading of 100 µL of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD using a portable sample preparation device was as good as those by conventional bench top protocol. It demonstrates that our novel centrifugal microfluidics platform enables a full integration of complex biological reactions that require multi-step fluidic control.

Graphical abstract: One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device

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Publication details

The article was received on 06 Nov 2006, accepted on 22 Jan 2007 and first published on 15 Feb 2007


Article type: Paper
DOI: 10.1039/B616115D
Citation: Lab Chip, 2007,7, 565-573
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    One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device

    Y. Cho, J. Lee, J. Park, B. Lee, Y. Lee and C. Ko, Lab Chip, 2007, 7, 565
    DOI: 10.1039/B616115D

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