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Issue 3, 2007
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A novel 3D mammalian cell perfusion-culture system in microfluidic channels

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Abstract

Mammalian cells cultured on 2D surfaces in microfluidic channels are increasingly used in drug development and biological research applications. These systems would have more biological or clinical relevance if the cells exhibit 3D phenotypes similar to the cells in vivo. We have developed a microfluidic channel based system that allows cells to be perfusion-cultured in 3D by supporting them with adequate 3D cell–cell and cell–matrix interactions. The maximal cell–cell interaction was achieved by perfusion-seeding cells through an array of micropillars; and 3D cell–matrix interactions were achieved by a polyelectrolyte complex coacervation process to form a thin layer of matrix conforming to the 3D cell shapes. Carcinoma cell lines (HepG2, MCF7), primary differentiated (hepatocytes) and primary progenitor cells (bone marrow mesenchymal stem cells) were perfusion-cultured for 72 hours to 1 week in the microfluidic channel, which preserved their 3D cyto-architecture and cell-specific functions or differentiation competence. This transparent 3D microfluidic channel-based cell culture system also allows direct optical monitoring of cellular events for a wide range of applications.

Graphical abstract: A novel 3D mammalian cell perfusion-culture system in microfluidic channels

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Publication details

The article was received on 16 Oct 2006, accepted on 21 Dec 2006 and first published on 23 Jan 2007


Article type: Paper
DOI: 10.1039/B614872G
Citation: Lab Chip, 2007,7, 302-309
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    A novel 3D mammalian cell perfusion-culture system in microfluidic channels

    Y. Toh, C. Zhang, J. Zhang, Y. M. Khong, S. Chang, V. D. Samper, D. van Noort, D. W. Hutmacher and H. Yu, Lab Chip, 2007, 7, 302
    DOI: 10.1039/B614872G

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