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Issue 2, 2007
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A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects

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Abstract

Practical HIV diagnostics are urgently needed in resource-limited settings. While HIV infection can be diagnosed using simple, rapid, lateral flow immunoassays, HIV disease staging and treatment monitoring require accurate counting of a particular white blood cell subset, the CD4+ T lymphocyte. To address the limitations of current expensive, technically demanding and/or time-consuming approaches, we have developed a simple CD4 counting microfluidic device. This device uses cell affinity chromatography operated under differential shear flow to specifically isolate CD4+ T lymphocytes with high efficiency directly from 10 microlitres of unprocessed, unlabeled whole blood. CD4 counts are obtained under an optical microscope in a rapid, simple and label-free fashion. CD4 counts determined in our device matched measurements by conventional flow cytometry among HIV-positive subjects over a wide range of absolute CD4 counts (R2 = 0.93). This CD4 counting microdevice can be used for simple, rapid and affordable CD4 counting in point-of-care and resource-limited settings.

Graphical abstract: A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects

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Publication details

The article was received on 19 Sep 2006, accepted on 02 Nov 2006 and first published on 24 Nov 2006


Article type: Paper
DOI: 10.1039/B612966H
Citation: Lab Chip, 2007,7, 170-178
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    A microfluidic device for practical label-free CD4+ T cell counting of HIV-infected subjects

    X. Cheng, D. Irimia, M. Dixon, K. Sekine, U. Demirci, L. Zamir, R. G. Tompkins, W. Rodriguez and M. Toner, Lab Chip, 2007, 7, 170
    DOI: 10.1039/B612966H

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