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Issue 2, 2007
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A linear dilution microfluidic device for cytotoxicity assays

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A two-layer polymer microfluidic device is presented which creates nine linear dilutions from two input fluid streams mixed in varying volumetric proportions. The linearity of the nine dilutions is conserved when the flow rate is held constant at 1.0 µl min−1 (R2 = 0.9995) and when it is varied from 0.5–16 µl min−1 (R2 = 0.9998). An analytical expression is presented for designing microfluidic devices with arbitrary numbers of linear dilutions. To demonstrate the efficacy of this device, primary human epidermal keratinocytes (HEK) were stained with nine dilutions of calcein, resulting in a linear spread of fluorescent intensities (R2 = 0.94). The operating principles of the device can be scaled up to incorporate any number of linear dilutions. This scalability, coupled with an intrinsic ability to create linear dilutions under a variety of operating conditions, makes the device applicable to high throughput screening applications such as combinatorial chemistry or cytotoxicity assays.

Graphical abstract: A linear dilution microfluidic device for cytotoxicity assays

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Publication details

The article was received on 26 Jun 2006, accepted on 28 Sep 2006 and first published on 19 Oct 2006

Article type: Paper
DOI: 10.1039/B608990A
Citation: Lab Chip, 2007,7, 226-232
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    A linear dilution microfluidic device for cytotoxicity assays

    G. M. Walker, N. Monteiro-Riviere, J. Rouse and A. T. O'Neill, Lab Chip, 2007, 7, 226
    DOI: 10.1039/B608990A

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