Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

Marco Malavolta; Francesco Piacenza; Laura Costarelli; Robertina Giacconi; Elisa Muti; Catia Cipriano; Silvia Tesei; Sandro Spezia; Eugenio Mocchegiani

doi:10.1039/B704577H

skip RSC | ChemSpider | Feedback

Home > Journals > Journal of Analytical A... > Combining UHR-SEC-HPLC-...

Authors & Referees | Librarians

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Journal of Analytical Atomic Spectrometry

Issue 9, 2007

More about this Journal
Editorial Board
Submit an Article

Follow Journal |

Journal Home | RSC Journals

Technical Note

Previous Article | Next Article

Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

Marco Malavolta , Francesco Piacenza , Laura Costarelli , Robertina Giacconi , Elisa Muti , Catia Cipriano , Silvia Tesei , Sandro Spezia and Eugenio Mocchegiani

J. Anal. At. Spectrom., 2007, 22, 1193-1198

DOI: 10.1039/B704577H
Received 28 Mar 2007, Accepted 07 Jun 2007
First published on the web 26 Jun 2007
This article is part of the collection: 2007 European Winter Conference on Plasma Spectrochemistry, Taormina, Italy

Please wait while Download options loads

Request Permissions

Abstract
Cited by
Related Content

A simple ultra high resolution (UHR)-size exclusion HPLC (SEC-HPLC) system with online detection by ICP-MS was set-up to quantify metallothioneins (MT), Zn bound to MT and to other cytosolic protein fractions with defined molecular weight in human peripheral blood mononuclear cells (PBMC). Applications of this method in combination with flow cytometry (FC) offer powerful diagnostic approaches for the evaluation of the zinc status and for quantifying MT in a very small sample amount.

Abstract from Mass Spectrometry Bulletin 2007

Conference, Inductively coupled plasma/MS, LC/MS, Man.

Abstract from Analytical Abstracts 2007

Articles By

Marco Malavolta
Francesco Piacenza
Laura Costarelli
Robertina Giacconi
Elisa Muti
Catia Cipriano
Silvia Tesei
Sandro Spezia
Eugenio Mocchegiani

Terms & Conditions|Privacy |Accessibility|ACAP Enabled

Journal of Analytical Atomic Spectrometry

Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

Log in (Free Access)

Log in (Subscriber Access)

Articles By