On-line coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICP-MS) for the detection of Fe in metalloproteins

Marta Garijo Añorbe; Jürgen Messerschmidt; Ingo Feldmann; Norbert Jakubowski

doi:10.1039/B704541G

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Journal of Analytical Atomic Spectrometry

Issue 8, 2007

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On-line coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICP-MS) for the detection of Fe in metalloproteins

Marta Garijo Añorbe , Jürgen Messerschmidt , Ingo Feldmann and Norbert Jakubowski

J. Anal. At. Spectrom., 2007, 22, 917-924

DOI: 10.1039/B704541G
Received 26 Mar 2007, Accepted 30 May 2007
First published on the web 20 Jun 2007
This article is part of the collection: Metallomics

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In this paper, we have coupled on-line gel electrophoresis to ICP-MS to investigate the separation of iron containing proteins (cytochrome C, haemoglobin, transferrin and ferritin). The GE system has been operated with quite different conditions, using (a) sodium dodecylsulfate (SDS) for charging and denaturing proteins and (b) using native conditions below and above the isoelectric point resulting in anodal and cathodal separations. The loss of the iron metal under each condition is discussed, so that quantification cannot be applied in each mode. For this purpose the ratio of ⁵⁶Fe⁺ to ³²S⁺ was additionally measured.

Abstract from Analytical Abstracts 2008

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Instrumental parameters for the ICP-MS system
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Journal of Analytical Atomic Spectrometry

On-line coupling of gel electrophoresis (GE) and inductively coupled plasma-mass spectrometry (ICP-MS) for the detection of Fe in metalloproteins

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