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Issue 9, 2007
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Nano-HPLC-inductively coupled plasma mass spectrometry for arsenic speciation

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Abstract

A nano-high performance liquid chromatography-inductively coupled plasma mass spectrometry (nano-HPLC-ICPMS) method is developed, using a demountable direct injection high efficiency nebulizer (d-DIHEN), to reduce sample and mobile phase consumption, minimize organic waste generation, decrease analysis time, and enhance separation efficiency. A HPLC column (50 mm × 0.3 mm id), packed with 3.5 μm C18 material, is explored for chromatographic separation of five arsenic species naturally present in the environment or introduced as a pollutant: sodium (meta)arsenite [As(III)], arsenic acid [As(V)], dimethylarsenic acid (DMA), disodium methylarsenate (MA), and p-arsanilic acid (p-ASA). A fast chromatographic separation of five arsenic species is achieved in less than 12 min at a solution flow rate of 0.9 μL min−1 using a 50 nL sample injection. The HPLC-ICPMS interface provides well defined flow injection profiles at various concentrations, giving a correlation coefficient of 0.999 for each individual arsenic species calibration curve. Precision values for peak height and area of five arsenic species range from 0.5 to 6.5% RSD and absolute detection limits are within 0.4 to 5.4 pg arsenic, which are comparable to previously reported data at higher solution uptake rates (20 μL min−1 to 1 mL min−1) and larger sample injection volumes (20–100 μL).

Graphical abstract: Nano-HPLC-inductively coupled plasma mass spectrometry for arsenic speciation

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Publication details

The article was received on 06 Mar 2007, accepted on 30 May 2007 and first published on 21 Jun 2007


Article type: Technical Note
DOI: 10.1039/B703257A
Citation: J. Anal. At. Spectrom., 2007,22, 1199-1205
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    Nano-HPLC-inductively coupled plasma mass spectrometry for arsenic speciation

    R. G. Brennan, S. E. O’Brien Murdock, M. Farmand, K. Kahen, S. Samii, J. M. Gray and A. Montaser, J. Anal. At. Spectrom., 2007, 22, 1199
    DOI: 10.1039/B703257A

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