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Issue 1, 2007
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Lactic acid production from waste sugarcane bagasse derived cellulose

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Abstract

Production of L(+)lactic acid from sugarcane bagasse cellulose, one of the abundant biomass materials available in India, was studied. The bagasse was chemically treated to obtain a purified bagasse cellulose sample, which is much more amenable to cellulase enzyme attack than bagasse itself. This sample, at high concentration (10%), was hydrolyzed by cellulase enzyme preparations (10 FPU g–1 cellulose) derived from mutants generated in our own laboratory. We obtained maximum hydrolysis (72%), yielding glucose and cellobiose as the main end products. Lactic acid was produced from this bagasse cellulose sample by simultaneous saccharification and fermentation (SSF) in a media containing a cellulase enzyme preparation derived from Penicillium janthinellum mutant EU1 and cellobiose utilizing Lactobacillus delbrueckii mutant Uc-3. A maximum lactic acid concentration of 67 g l–1 was produced from a concentration of 80 g l–1 of bagasse cellulose, the highest productivity and yield being 0.93 g l–1 h–1 and 0.83 g g–1, respectively. The mutant Uc-3 was found to utilize high concentrations of cellobiose (50 g l–1) and convert it into lactic acid in a homo-fermentative way. Considering that bagasse is a waste material available in abundance, we propose to valorize this biomass to produce cellulose and then sugars, which can be fermented to products such as ethanol and lactic acid.

Graphical abstract: Lactic acid production from waste sugarcane bagasse derived cellulose

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Publication details

The article was received on 24 Apr 2006, accepted on 04 Oct 2006 and first published on 17 Oct 2006


Article type: Paper
DOI: 10.1039/B605839F
Citation: Green Chem., 2007,9, 58-62
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    Lactic acid production from waste sugarcane bagasse derived cellulose

    M. G. Adsul, A. J. Varma and D. V. Gokhale, Green Chem., 2007, 9, 58
    DOI: 10.1039/B605839F

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