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Issue 6, 2007
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Real-time PCR for detection of the Aspergillus genus

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Abstract

Aspergillus is a genus of mold that has strong indoor sources, including several species capable of acting as opportunistic pathogens. Previous studies suggest that Aspergillus could serve as an indicator for abnormal mold growth or moisture, making it an important genus for environmental monitoring. Here, a quantitative polymerase chain reaction (qPCR, or real-time PCR) assay is presented for Aspergillus. The assay shows good specificity for the genus, detecting all Aspergillus species tested, although a few non-Aspergillus species are also amplified. Sensitivity testing demonstrates that DNA representing one conidium can be detected. A validation study compared qPCR results against direct microscopy counts using A. fumigatus conidia aerosolized into a laboratory chamber. The assay was then used to quantify Aspergillus in indoor air samples, demonstrating its utility for environmental monitoring. Analysis of a small number of clinical sputum samples showed complete agreement with culturing results.

Graphical abstract: Real-time PCR for detection of the Aspergillus genus

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Publication details

The article was received on 03 Jan 2007, accepted on 02 Apr 2007 and first published on 25 Apr 2007


Article type: Paper
DOI: 10.1039/B618937G
Citation: J. Environ. Monit., 2007,9, 599-609
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    Real-time PCR for detection of the Aspergillus genus

    M. D. Goebes, L. M. Hildemann, E. Kujundzic and M. Hernandez, J. Environ. Monit., 2007, 9, 599
    DOI: 10.1039/B618937G

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