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Issue 37, 2007
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Fluorescence of the DNA double helices (dAdT)n·(dAdT)n studied by femtosecond spectroscopy

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Abstract

Polymeric and oligomeric DNA helices, poly(dAdT)·poly(dAdT) and (dAdT)10·(dAdT)10, composed of 200–400 and 20 adeninethymine base pairs, respectively, are studied by fluorescence upconversion. Fluorescence decays, anisotropy decays and time-resolved spectra, obtained for this alternating base sequence, are compared with those determined previously for the homopolymeric sequence (dA)n·(dT)n. It is shown that identical fluorescence decays may correspond to quite different anisotropy decays and vice versa, both varying with the emission wavelength, the base sequence and the duplex size. Our observations cannot be explained in terms of monomer and excimer emission exclusively, as concluded in the past on the basis of steady-state measurements. Excitons also contribute to the fluorescence. These are rapidly trapped by excimers, characterized by long-lived weak emission.

Graphical abstract: Fluorescence of the DNA double helices (dAdT)n·(dAdT)n studied by femtosecond spectroscopy

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Publication details

The article was received on 24 May 2007, accepted on 12 Jul 2007 and first published on 31 Jul 2007


Article type: Paper
DOI: 10.1039/B707882J
Citation: Phys. Chem. Chem. Phys., 2007,9, 5143-5148
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    Fluorescence of the DNA double helices (dAdT)n·(dAdT)n studied by femtosecond spectroscopy

    D. Onidas, T. Gustavsson, E. Lazzarotto and D. Markovitsi, Phys. Chem. Chem. Phys., 2007, 9, 5143
    DOI: 10.1039/B707882J

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