Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 16, 2007
Previous Article Next Article

Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance

Author affiliations

Abstract

The biological function of protein, DNA, and RNA molecules often depends on relative movements of domains with dimensions of a few nanometers. This length scale can be accessed by distance measurements between spin labels if pulsed electron paramagnetic resonance (EPR) techniques such as electron–electron double resonance (ELDOR) and double-quantum EPR are used. The approach does not require crystalline samples and is well suited to biomacromolecules with an intrinsic flexibility as distributions of distances can be measured. Furthermore, oligomerization or complexation of biomacromolecules can also be studied, even if it is incomplete. The sensitivity of the technique and the reliability of the measured distance distribution depend on careful optimization of the experimental conditions and procedures for data analysis. Interpretation of spin-to-spin distance distributions in terms of the structure of the biomacromolecules furthermore requires a model for the conformational distribution of the spin labels.

Graphical abstract: Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 13 Oct 2006, accepted on 15 Dec 2006 and first published on 23 Jan 2007


Article type: Invited Article
DOI: 10.1039/B614920K
Citation: Phys. Chem. Chem. Phys., 2007,9, 1895-1910
  •   Request permissions

    Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance

    G. Jeschke and Y. Polyhach, Phys. Chem. Chem. Phys., 2007, 9, 1895
    DOI: 10.1039/B614920K

Search articles by author